Experimental modelling of full-thickness skin wounds in pigs

  • Received 11.10.2024

  • Revised 04.01.2025

  • Accepted 25.02.2025



  • 776 Views

  • Retrieved from Vol. 7, No. 1, 2025

  • Pages 43-50

In the modern context, there is a growing need to develop a relevant experimental model of a skin wound that closely replicates the regeneration processes occurring in human wounds. The aim of this study was to develop a method to prevent premature contraction of wound edges during the experimental modelling of a full-thickness skin wound in pigs, thereby creating optimal conditions for evaluating the effectiveness of local treatment approaches. An experimental study was conducted on a white pig weighing 15 kg. A full-thickness skin wound measuring 5×5 cm was created on the animal’s back under thiopental sodium anaesthesia at a dosage of 80 mg/kg. Tissue samples were collected from the wound site via punch biopsy under general anaesthesia, fixed in 10% neutral formalin, and embedded in paraffin using standard histological techniques. Deparaffinised sections were stained with haematoxylin and eosin. A computer program was developed in Python to calculate the wound area using the Monte Carlo method. To visualise the results and observe trends, graphical representations in the form of diagrams were used. The study demonstrated the feasibility of modulating contraction in full-thickness skin defects by applying incisions. The most effective method involved tangential incisions at each corner of the wound, each measuring up to 1 cm in length. This technique reduced the degree of wound edge contraction. On day 28 of observation, the wound area in the experimental group was 69.3% of the original size, compared to 39.3% in the control group. To accurately assess the effectiveness of treatments for full-thickness skin wounds in porcine models, it is essential to maintain a wound of appropriate size for at least 28 days to allow for observation of scar tissue formation. The proposed wound model enables controlled modulation of contraction and preserves an adequate wound surface area for the duration necessary to study scar formation processes

excisional wound model; porcine model; wound edge contraction; wound area; wound healing; dermal matrix

https://doi.org/10.63341/bmbr/1.2025.43

[1] Hamilton DW, Walker JT, Tinney D, Grynyshyn M, El-Warrak A, Truscott E, et al. The pig as a model system for investigating the recruitment and contribution of myofibroblasts in skin healing. Wound Rep Reg. 2022;30(1):45–63. DOI: 10.1111/wrr.12981

[2] Ryk T. Molecular genetic status of pigs of Ukrainian breeds suitable for use in xenotranplantation. Sci Rep Natl Univ Life Environ Sci Ukr. 2024;20(2). DOI: 10.31548/dopovidi.2(108).2024.003

[3] Shiff J, Schwartz K, Hausman B, Seshadri DR, Bogie KM. Development and use of a porcine model with clinically relevant chronic infected wounds. J Tissue Viability. 2023;32(4):527–35. DOI: 10.1016/j.jtv.2023.08.004

[4] Elloso M, Hutter MF, Jeschke N, Rix G, Chen Y, Douglas A, et al. Challenges of porcine wound models: A review. Int J Transl Med. 2025;5(1):4. DOI: 10.3390/ijtm5010004

[5] Tucci M, Hildebrandt D, Lichtenhan J, Benghuzzi H. Evaluation of full thickness wounds following application of a visco-liquid hemostat in a swine model. Pathophysiology. 2024;31(3):458–70. DOI: 10.3390/pathophysiology31030034

[6] Diller RB, Tabor AJ. The role of the extracellular matrix (ECM) in wound healing: A review. Biomimetics. 2022;7(3):87. DOI: 10.3390/biomimetics7030087

[7] Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics. 2020;12(8):735. DOI: 10.3390/pharmaceutics12080735

[8] Bargavi P, Ramya R, Chitra S, Vijayakumari S, Chandran RR, Durgalakshmi D, et al. Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications. Biomater Sci. 2020;8:4003–25. DOI: 10.1039/D0BM00714E 

[9] Xu J, Fang H, Zheng S, Li L, Jiao Z, Wang H, et al. A biological functional hybrid scaffold based on decellularized extracellular matrix/gelatin/chitosan with high biocompatibility and antibacterial activity for skin tissue engineering. Int J Biol Macromol. 2021;187:840–9. DOI: 10.1016/j.ijbiomac.2021.07.162

[10] Dai C, Shih S, Khachemoune A. Skin substitutes for acute and chronic wound healing: An updated review. J Dermatolog Treat. 2020;31(6):639–48. DOI: 10.1080/09546634.2018.1530443

[11] Kuo TY, Huang CC, Shieh SJ, Wang YB, Lin MJ, Wu MC, et al. Skin wound healing assessment via an optimized wound array model in miniature pigs. Sci Rep. 2022;12(1):445. DOI: 10.1038/s41598-021-03855-y

[12] Order of the Ministry of Health of Ukraine No. 944. On Approval of the Procedure for Conducting Preclinical Studies of Medicinal Products and Expertise of Materials of Preclinical Studies of Medicinal Products [Internet]. 2009 December 14 [cited 2024 October 7]. Available from: https://ips.ligazakon.net/document/view/re17348?an=2&ed=2009_12_14

[13] European Convention for the Protection of Vertebrate Animals Used for Research and Other Scientific Purposes [Internet]. 1986 March 18 [cited 2024 October 7]. Available from: https://zakon.rada.gov.ua/laws/show/994_137#Text

[14] Patrieva L, Pidpala T, Kalynychenko H. Bioethics guidelines. Mykolaiv: Mykolaiv National Agrarian University; 2021. P. 125.

[15] Law of Ukraine No. 3447-IV. On the Protection of Animals from Cruelty [Internet]. 2006 February 21 [cited 2024 October 7]. Available from: https://zakon.rada.gov.ua/laws/show/3447-15#Text

[16] Gould H, Tobochnik J, Harrison DE. An introduction to computer simulation methods: Applications to physical systems part 1 and part 2. Comput Phys. 1988;2(1):90–1. DOI: 10.1063/1.4822668

[17] Elia R, Maruccia M, Di Summa PG, Trisciuzzi R, Lovero G, Cazzato G, et al. Conventional versus regenerative methods for wound healing: A comparative experimental study on a sheep model. Medicina. 2024;60(11):1836. DOI: 10.3390/medicina60111836

[18] Coger V, Million N, Rehbock C, Sures B, Nachev M, Barcikowski S, et al. Tissue concentrations of zinc, iron, copper, and magnesium during the phases of full thickness wound healing in a rodent model. Biol Trace Elem Res. 2019;191(1):167–76. DOI: 10.1007/s12011-018-1600-y

[19] Udegbunam SO, Ogbobe S, Okereke NH, Enejere AS, Udegbunam IR, Ezeobialu TH. Assessment of wound contraction, re-epithelialization and histological changes in full thickness excision wounds of rats treated with different concentrations of hydrogen peroxide. Trop J Pharm Res. 2021;20(8):1623–9. DOI: 10.4314/tjpr.v20i8.11

[20] Wallace HA, Basehore BM, Zito PM. Wound healing phases. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2022. P. 35–43.

[21] Cross SE, Naylor IL, Coleman RA, Teo TC. An experimental model to investigate the dynamics of wound contraction. Br J Plast Surg. 1995;48(4):189–97. DOI: 10.1016/0007-1226(95)90001-2

[22] Masson-Meyers DS, Andrade TAM, Caetano GF, Guimaraes FR, Leite MN, Leite SN, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol. 2020;101(1–2):21–37. DOI: 10.1111/iep.12346

[23] Park SA, Raghunathan VK, Shah NM, Teixeira L, Motta MJ, Covert J, et al. PDGF-BB does not accelerate healing in diabetic mice with splinted skin wounds. PLoS One. 2014;9(8):e104447. DOI: 10.1371/journal.pone.0104447

[24] Wang X, Ge J, Tredget EE, Wu Y. The mouse excisional wound splinting model, including applications for stem cell transplantation. Nat Protoc. 2013;8(2):302–9. DOI: 10.1038/nprot.2013.002

[25] Karppinen SM, Heljasvaara R, Gullberg D, Tasanen K, Pihlajaniemi T. Toward understanding scarless skin wound healing and pathological scarring. F1000Res. 2019;8:787. DOI: 10.12688/f1000research.18293.1

[26] Chang F, Yan L, Zha Y, Hong X, Zhu K, Fei Y, et al. Development of a wound epithelialization healing model: Reducing the impact of contraction healing on the wound surface. J Burn Care Res. 2024;45(4):1016–25. DOI: 10.1093/jbcr/irae065