Comparative analysis of statistical data and oral hygiene indices among different groups of smokers

The aim of the study was to compare the oral hygiene status of individuals consuming different types of tobacco products. The research methodology involved stratification of the sample by type of tobacco use, clinical examination employing standardised indices, comparative statistical analysis, and visual verification of the outcomes. The study was conducted from September 2023 to April 2024 at the private dental clinic of sole proprietor Kravchenko B.I. “GooDDentist”. The highest value of the decayed, missing, and filled teeth index was recorded among cigarette smokers (13.0 points), whereas users of heat-not-burn systems exhibited the greatest levels of the soft plaque index (3.05) and calculus index (2.90), indicating intensive biofilm accumulation. Consumers of smokeless (application) tobacco demonstrated the highest gingival index (3.60), reflecting gingival irritation. The proportion of each group in the overall clinical cohort comprised 41.7% (cigarette smokers), 36.1% (users of heat-not-burn systems), and 37.2% (pouch tobacco users). The most clinically relevant adjuncts were the disclosing agent (96%), remineralising gel (94%), and glycine-based air-abrasive powder (92%), confirmed by both objective indicators and patient-reported outcomes. Clinical profiles of tobacco users in Ukraine corresponded to trends documented in Germany, Lithuania, and the Czech Republic, where inflammatory, biofilm-related, or caries-associated alterations predominate depending on the type of tobacco exposure. The findings support the feasibility of implementing personalised preventive protocols tailored to the specific tobacco product type, which may be integrated into dental and clinical hygiene practice as well as inform the development of state-level guidelines for individualised prevention of tobacco-associated oral lesions

tobacco use; clinical risk; calculus index; dental prevention; smokeless tobacco; enamel lesions

https://doi.org/10.63341/bmbr/4.2025.08
  1. Krawczyk-Suszek M, Kleinrok A. Health-related quality of life (HRQoL) of people over 65 years of age. Int J Environ Res Public Health. 2022;19(2):625. DOI: 10.3390/ijerph19020625
  2. Antinozzi M, Giffi M, Sini N, Gallè, F, Valeriani F, De Vito C, et al. Cigarette smoking and human gut microbiota in healthy adults: A systematic review. Biomedicines. 2022;10(2):510. DOI: 10.3390/biomedicines10020510
  3. Baumeister SE, Listl S, Holtfreter B, Nascimento GG, Leite FR. Causal effect of smoking and cessation on tooth loss. J Clin Periodontol. 2025;52(4):539–46. DOI: 10.1111/jcpe.14106
  4. Ibrahim HF, Hassan GS. Qualitative and quantitative assessment of the potential effect of cigarette smoking on enamel of human smokers’ teeth. Arch Oral Biol. 2021;121:104953. DOI: 10.1016/j.archoralbio.2020.104953
  5. Beklen A, Sali N, Yavuz MB. The impact of smoking on periodontal status and dental caries. Tob Induced Diseases. 2022;20:72. DOI: 10.18332/tid/152112
  6. Karanjkar RR, Preshaw PM, Ellis JS, Holliday R. Effect of tobacco and nicotine in causing staining of dental hard tissues and dental materials: A systematic review and meta-analysis. Clin Exp Dent Res. 2023;9(1):150–64. DOI: 10.1002/cre2.683
  7. Souto MLS, Rovai ES, Villar CC, Braga MM, Pannuti CM. Effect of smoking cessation on tooth loss: A systematic review with meta-analysis. BMC Oral Health. 2019;19:245. DOI: 10.1186/s12903-019-0930-2
  8. Lee SH, Cha JY, Choi SH, Kim BI, Cha JK, Hwang CJ. Effect of nicotine on orthodontic tooth movement and bone remodeling in rats. Korean J Orthod. 2021;51(4):282–92. DOI: 10.4041/kjod.2021.51.4.282
  9. Dvornyk AV. Clinical and experimental justification of the choice of teeth whitening methods and assessment of their effectiveness [PhD dissertation]. Poltava: Poltava State Medical University; 2023.
  10. Yashkina TO. Clinical and functional status and sociological portrait of healthy young people who use alternative types of tobacco smoking [PhD dissertation]. Dnipro: Dnipro State Medical University; 2023.
  11. Korzhov I, Geleta D. Harm of snus and its consequences. In: V international student scientific conference “Development of society and science in the conditions of digital transformation”. Vinnytsia: LLC “Ukrlogos Group”; 2024. P. 118–9.
  12. Komisova T, Kovalenko L, Klymenko O, Osynskyi M. Assessment of the impact of electronic cigarettes on the respiratory system of high school students. In: 3rd international scientific and practical conference “Natural science and education: Current state and development prospects”. Kharkiv: Kharkiv National Pedagogical University named after H.S. Skovoroda; 2022. P. 64–6.
  13. WMA Declaration of Helsinki – Ethical Principles for Medical Research Involving Human Participants [Internet]. [cited 2025 April 15]. Available from: https://www.wma.net/policies-post/wma-declaration-of-helsinki-ethical-principles-for-medical-research-involving-human-subjects/
  14. Ministry of Health of Ukraine Order No. z1010-09. On Approval of the Procedure for Conducting Clinical Trials of Medicinal Products and Examination of Clinical Trial Materials and the Model Regulation on Ethics Committees [Internet]. 2009 September 23 [cited 2025 April 15]. Available from: https://zakon.rada.gov.ua/laws/show/z1010-09#Text
  15. World Health Organization. Tobacco cessation and oral health integration. Geneva: World Health Organization; 2017. 72 P.
  16. World Health Organization. Tobacco Germany 2023 country profile [Internet]. 2023 July 1 [cited 2025 April 14]. Available from: https://www.who.int/publications/m/item/tobacco-deu-2023-country-profile
  17. World Health Organization. Tobacco Czechia 2023 country profile [Internet]. 2023 July 1 [cited 2025 April 14]. Available from: https://www.who.int/publications/m/item/tobacco-cze-2023-country-profile
  18. World Health Organization. Tobacco Lithuania 2023 country profile [Internet]. 2023 July 1 [cited 2025 April 15]. Available from: https://www.who.int/publications/m/item/tobacco-ltu-2023-country-profile
  19. World Health Organization. Tobacco control in the WHO European Region [Internet]. 2020 October 1 [cited 2025 April 15]. Available from: https://www.who.int/europe/publications/i/item/WHO-EURO-2020-5615-45380-64938
  20. Greene JG, Vermillion JR. The simplified oral hygiene index. J American Dent Association. 1964;68(1):7–13. DOI: 10.14219/jada.archive.1964.0034
  21. Fischman SL. Clinical index systems used to assess the efficacy of mouth‐rinses on plaque and gingivitis. J Clin Periodont. 1988;15(8):506–10. DOI: 10.1111/j.1600-051x.1988.tb01022.x
  22. Shapiro SS, Wilk MB. An analysis of variance test for normality (complete samples). Biometrika. 1965;52(3–4):591–611. DOI: 10.2307/2333709
  23. Levene H. Robust tests for equality of variances. In: Olkin I, Fhurye SG, Hoeffding W, Madow WG, Mann HB, editors. Contributions to probability and statistics: Essays in honor of Harold Hotelling. Stanford: Stanford University Press; 1960. P. 278–92.
  24. Tukey JW. Comparing individual means in the analysis of variance. Biometrics. 1949;5(2):99–114. DOI: 10.2307/3001913
  25. Veiga N, Figueiredo R, Correia P, Lopes P, Couto P, Fernandes GVO. Methods of primary clinical prevention of dental caries in the adult patient: An integrative review. Healthcare. 2023;11(11):1635. DOI: 10.3390/healthcare11111635
  26. Henschke C, Winkelmann J, Eriksen A, Pérez EO, Klingenberger D. Oral health status and coverage of oral health care: A five-country comparison. Health Policy. 2023;137:104913. DOI: 10.1016/j.healthpol.2023.104913
  27. Petrauskienė S, Žemaitienė M, Bendoraitienė EA, Saldūnaitė-Mikučionienė K, Vasiliauskienė I, Zūbienė J, et al. A cross-sectional study of oral health status and behavioral risk indicators among non-smoking and currently smoking lithuanian adolescents. Int J Environ Res Public Health. 2023;20(16):6609. DOI: 10.3390/ijerph20166609
  28. Panariello B, Panariello FD, Misir A, Barboza EP. An umbrella review of e-cigarettes’ impact on oral microbiota and biofilm buildup. Pathogens. 2025;14(6):578. DOI: 10.3390/pathogens14060578
  29. Lara-Muñoz MDC, Torres-Escobar ID, Espinosa De Santillana IA, García-Solano M, Muñoz-Quintana G, Martínez-Hernández AM, et al. Association of tobacco and alcohol consumption with self-reported tooth loss among teenagers and young adults: A large-scale, cross-sectional study. J Substance Use. 2023;28(6):962–7. DOI: 10.1080/14659891.2022.2114390
  30. Shah R, Singh A, Singh HM, Gupta R, Ghosh S. Effect of tobacco smoking on the periodontal health in Parsa, Nepal: A comparative cross-sectional study. J Oral Res Rev. 2024;16(1):1–5. DOI: 10.4103/jorr.jorr_29_23
  31. Gajendra S, McIntosh S, Ghosh S. Effects of tobacco product use on oral health and the role of oral healthcare providers in cessation: A narrative review. Tob Induc Dis. 2023;21:12. DOI: 10.18332/tid/157203
  32. Chaffee BW, Couch ET, Vora MV, Holliday RS. Oral and periodontal implications of tobacco and nicotine products. Periodontology 2000. 2021;87(1):241–53. DOI: 10.1111/prd.12395
  33. Ilchyshyn M, Furdychko A, Barylyak A, Fedun I, Gan I. Features of the influence of tobacco heating systems (GLO and IQOS) on the oral tissues condition. Ukr J Med Biol Sport. 2020;5(6):247–52. DOI: 10.63341/ujmbs/6.2020.247
  34. Camoni N, Conti G, Esteves-Oliveira M, Carvalho TS, Roccuzzo A, Cagetti MG, et al. Electronic cigarettes, heated tobacco products, and oral health: A systematic review and meta-analysis. Appl Sci. 2023;13(17):9654. DOI: 10.3390/app13179654
  35. Souto MLS, Carrer FCDA, Braga MM, Pannuti CM. Smoking cessation therapy is a cost-effective intervention to avoid tooth loss in Brazilian subjects with periodontitis: An economic evaluation. BMC Oral Health. 2021;21:616. DOI: 10.1186/s12903-021-01932-2
  36. Milleman J, Bosma ML, McGuire JA, Sunkara A, McAdoo K, DelSasso A, et al. Comparative effectiveness of toothbrushing, flossing and mouthrinse regimens on plaque and gingivitis: A 12-week virtually supervised clinical trial. J Dent Hygiene. 2022;96(3):21–34.
  37. Kumar S, Kumar A, Gupta A, Singh SK, Gupta A, Mehta P. Assessment of the relationship between oral health behavior, oral hygiene, and gingival status of adolescent tobacco consumers in ranchi, jharkhand: A comparative study. Adv Prev Med. 2021;2021(1):3548132. DOI: 10.1155/2021/3548132
  38. Ahad A, Bey A, Khan S, Ahmad MS. Periodontal status associated with dual habits of smoking and smokeless tobacco use: A cross-sectional study in young adults. J Adv Periodontol Implant Dent. 2021;13(2):69–75. DOI: 10.34172/japid.2021.010
  39. Hernández-Morales A, González-López BS, Scougall-Vilchis RJ, Bermeo-Escalona JR, Velázquez-Enríquez U, Islas-Zarazúa R, et al. Lip and oral cavity cancer incidence and mortality rates associated with smoking and chewing tobacco use and the human development index in 172 countries worldwide: An ecological study 2019-2020. Healthcare. 2023;11(8):1063. DOI: 10.3390/healthcare11081063
  40. Ford PJ, Rich AM. Tobacco use and oral health. Addiction. 2021;116(12):3531–40. DOI: 10.1111/add.15513
  41. Jebril A, Jarad F, Butterworth CJ, Bebb K. Assessment of oral disease burden among head and neck cancer patients in the Merseyside region. Br Dent J. 2024. DOI: 10.1038/s41415-024-8060-x
  42. World Health Organization. Global oral health status report: Towards universal health coverage for oral health by 2030 [Internet]. 2022 November 18 [cited 2025 April 14]. Available from: https://www.who.int/publications/i/item/9789240061484
  43. Neuberger M. Tobacco and alternative nicotine products and their regulation. In: Reichl F-X, Schwenk M, editors. Regulatory toxicology. Cham: Springer; 2021. P. 1127–51. DOI: 10.1007/978-3-030-57499-4_124
  44. D’Ambrosio F, Pisano M, Amato A, Iandolo A, Caggiano M, Martina S. Periodontal and peri-implant health status in traditional vs. heat-not-burn tobacco and electronic cigarettes smokers: A systematic review. Dent J. 2022;10(6):103. DOI: 10.3390/dj10060103