Microscopic changes in the neurocytes of the cerebellar cortex in dynamics under conditions of experimental thermal injury
Summary. Thermal injury as a stress factor of exogenous origin, in which the development of multiple organ failure and dysfunction of organs and systems of the body occur. The nervous system is one of the first to perceive and respond to pain impulses. It is important to reorganize all the structural components of the microcirculatory tract and neurons as a result of endogenous intoxication, which develops during thermal trauma. The target of lesions is the cerebellum, as a multifunctional organ of the central nervous system. The aim of the study – to establish the microscopic reorganization of neurocytes of the cerebellar cortex in the dynamics under conditions of experimental thermal trauma to the skin. Materials and Methods. The experimental study was performed on 24 adult white male rats weighing 180–200 g. Simulation of third-degree burns was performed with copper plates heated in boiled water to a temperature of 97–100º on the epilated surface of the skin of animal bodies under thiopental sodium. The size of the affected areas was 18–20 % of the body of rats. Collection of material for microscopic examination was performed in accordance with generally accepted methods. Histological sections obtained on a sled microtome 5–6 μm thick were stained with hematoxylin and eosin, toluidine blue by the method of Nissl. Semi-thin sections made on an ultramicrotome LKB-3 were stained with methylene blue. The micropreparations were studied using a MICROmed SEO SCAN light microscope and photo-documented using a Vision CCD Camera with a histological image output system. Results. Microscopic studies have shown an interdependence between the duration of the experiment and abnormalities in the structural components of neurons in all layers of the cerebellar cortex. In the early stages of the experiment there are reactive changes, which are adaptive-compensatory in nature with initial destructive damage. On day 14, and especially after 21 days, polymorphism is observed, significant cell degeneration, manifested by pycnosis of the nuclei, the development of intra- and pericellular edema in neurons of the granular, ganglionic and granular layers of the cerebellar cortex
cerebral cortex, neurocytes, Purkinje cells, thermal trauma, microscopic changes
https://doi.org/10.11603/bmbr.2706-6290.2021.2.12340[1] Ye H, De S. Thermal injury of skin and subcutaneous tissues: A review of experimental approaches and numerical models. Burns. 2017;43(5): 909-32. DOI: 10.1016/j.burns.2016.11.014.
[2] Kovalchuk OI. [Athogenesis of burn diease: mod-ern aspects]. Nauk. visnyk NMU im. O.O. Bohomoltsia. 2013;2(41): 64-9. Ukrainian.
[3] Pruitt BA, Wolf SE. An historical perspective on ad-vance in burn care over the past 100 years. Clin Plast Surg. 2009;36: 527-45. DOI: 10.1016/j.cps.2009.05.007
[4] Liu A, Ocotl E, Karim A, Wolf JJ, Cox BL, Eliceiri KW, et al. Modelling early thermal injury using an ex vivo human skin model of contact burns. Burns. 2020;47(3): 611-20. DOI: 10.1016/j.burns.2020.08.011
[5] Kramar SB, Volkov KS, Nebesna ZM. Morphometric studies of the damaged skin area afterexperimental thermal trauma and during correction with cryo-lyophilized xenograft skin substrate. Reports of Morphology. 2018;24(4): 22-8.DOI: 10.31393/morphology-journal-2018-24(4)-03.
[6] Kramar SB, Volkov KS, Lytvynyuk SO. [Microscopic and histochemical changes of the skin after experimental thermal trauma and аpplication of crioliofilized xenograft-skin subst rate]. Visnyk morfolohii. 2015;1(1): 6-9. Ukrainian.
[7] Nebesna ZM, Volkov KS, Bodnar PYa ., Shuturma OYa ., Kramar SB. Submicroscopic changes of the respiratoryalveolar macrophages at the late period after experimentalthermal injury and in combineduse of lyophilized xenograft substrate and surfactant. World of Medicine and Biology. 2018;2(64): 165-9.
[8] Getmanyuk IB, Nebesna ZM, Lykhatskyi PG, Shuturma OYa. [Рeculiarities of atrial cardiomyocytes’ ultrastructural reorganization at experimental thermal injury and in conditions of application of lyophilized xenograft substrate]. Visnyk naukovykh doslidzhen. 2019;2(95): 105-9. doi 10.11603/2415-8798.2019.2.10273. Ukrainian.
[9] Bilash SM, Pronina OM, Kononov BS. [Modern rep-resentations about processes of re-modeling of structural components of the cerebellum under conditions of action of the complex of chemicals]. Visnyk problem biolohii i medytsyny. 2020;1(155): 20-5. DOI: 10.29254/2077-4214-2020-1-155-20-25. Ukrainian.
[10] Coert JH. Pathophysiology of nerve regeneration and nerve reconstruction in burned patients. Burns. 2010;36(5): 593-8. DOI: 10.1016/j.burns.2009.10.007
[11] Halm M-P, Poquin D, Lestaevel P, Chancerelle Y, Graff C. Brain and cognitive impairments from burn injury in rats. Burns. 2006;32(5): 570-6. DOI: 10.1016/j.burns.2005.12.005.
[12] Lytvynyuk SO. Morphofunctional modifications of neurocytes of the rat hippocampal CA3 area after experi-mental thermal trauma. Neurophysiology. 2016;48: 399-406.
[13] Bekesevych AM. [Morphological features of the structure of the cerebellar cortex of rats in the norm and under prolonged exposure to the opioid]. Zaporozh med zhurn. 2015;3(90): 82-85. doi:10.14739/2310-1210.2015.3.44505. Ukrainian.
[14] Horalskyi LP, Khomych VT, Kononskyi OI. Fundamentals of histological technique and morphofunctionalmethods of research in normal and pathology. [Основи гістологічної техніки і морфофункціональні методи досліджень у нормі та при патології] Zhytomyr: Polissia; 2011. Ukrainian.