Features of changes in some parameters of the cellular and humoral links of the immune system under the conditions of experimental reproduction of allergic alveolitis during immobilization stress

Mykhailo S. Regeda; V.V.  Galii-Lutska

Sumarry. To date, the issue of finding out the specifics of changes in the immune system under the conditions of allergic alveolitis and immobilization stress remains unexplored, since these diseases are quite widespread. The aim of the study –to determine the nature of changes in the levels of T- and B-lymphocytes, circulating immune complexes (CIC) of the blood of male guinea pigs (GP) under conditions of allergic alveolitis (EAA), immobilization stress (IS) Materials and Methods. 100 male GP weighing 180-210 g were used, divided into 4 experimental groups (EG): 1 EG – intact GP; 2 EG – GP with EAA; 3 EG – GP with IS; 4 EG – GP with combination of EAA and IS. Animals were removed from the experiment on the days 1, 14 and 24 from the beginning of the study, according to such stages of development of IS as anxiety, resistance, exhaustion. Results. No significant changes were observed after 1 day of experimental reproduction of EAA. A decrease in the content of T-lymphocytes by 27.75 % (p<0.01) and 31.78 % (p<0.01), an increase in the level of B-lymphocytes by 19.87 % (p<0.01) and 27.15 % (p<0.01) and augmentation of CIC – by 36.98 % (p<0.01) and 40.89 % (p<0.01) respectively, relative to the values of the parameters of intact animals, was noted on the days 14 and 24 of research. A sharp decrease in T-lymphocytes by 30.72 % (p<0.01), 28.81 % (p<0.01) and 29.87 % (p<0.01), an increase in B-lymphocytes by 25.17 % (p<0.01), 19.21 % (p<0.01) and 15.23 % (p<0.01) and CIC by 39.58 % ( p<0.01), 33.85 % (p<0.01) and 30.47 % (p<0.01), compared to the control group was observed during the simulation of IS on the above-mentioned days of the experiment respectively. There was a progressive decrease in the level of T-lymphocytes by 30.72 % (p<0.01), 28.81 % (p<0.01) and 29.87 % (p<0.01), an increase in B-lymphocytes by 27.82 % (p<0.01), 31.13 % (p<0.01) and 44.37 % (p<0.01) and CIC by 41.15 % (p<0.01), 46.62 % (p<0.01) and 50.78 % (p<0.01) on the above-mentioned days of the experiment, respectively under the combination of EAA and IS. Conclusions. Experimental allergic alveolitis combined with immobilization stress is accompanied by a decrease in the content of T-lymphocytes and an increase in the level of CIC and B-lymphocytes in the blood, which indicates a pronounced suppression of the cellular and activation of the humoral link of the immune system in EAA and IS especially on the 24th day of the experiment

allergic alveolitis, immobilization stress, T-lymphocytes, B-lymphocytes, circulating immune complexes

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